Optimization of Phenolic Compounds Extraction from Flax Shives and Their Effect on Human Fibroblasts

Table 3

Analysis of influence of solvent volume and number of extractions on phenylpropanoids extraction yield in flax shives. For the determination of statistical significance ANOVA test was used ().

(a)

30%	Extraction I (µg/g)	Extraction II (µg/g)	Extraction III (µg/g)
4-Hydroxybenzoic acid	4.8 ± 0.62	1.81 ± 0.15	0.8 ± 0.06
Vanillic acid	10.45 ± 1.46	3.13 ± 0.89	0.75 ± 0.17
Vanillin	66 ± 9.91	16.11 ± 3.31	2.8 ± 0.21
p-Coumaric acid	18.16 ± 2.14	3.84 ± 0.84	0.64 ± 0.21
Syringaldehyde	12.02 ± 1.34	3.2 ± 0.48	0.59 ± 0.06
Acetovanillone	3.39 ± 0.49	0.84 ± 0.16	0 ± 0
Ferulic acid	16.79 ± 2.15	3.71 ± 0.55	0.68 ± 0.13
Total	131.61	32.64	6.26

(b)

50%	Extraction I (µg/g)	Extraction II (µg/g)	Extraction III (µg/g)
4-Hydroxybenzoic acid	5.72 ± 1.0	1.74 ± 0.1	0.44 ± 0.06
Vanillic acid	11.2 ± 0.6	2.41 ± 0.3	0.45 ± 0.06
Vanillin	±	12.91 ± 3	1.45 ± 0.5
p-Coumaric acid	±	2.89 ± 0.9	0.3 ± 0.1
Syringaldehyde	±	2.42 ± 0.6	0.31 ± 0.1
Acetovanillone	3.92 ± 0.4	0.55 ± 0.1	0.1 ± 0.03
Ferulic acid	17.06 ± 1.0	2.97 ± 0.4	0.26 ± 0.0
Total	142.89	25.89	3.31

(c)

100%	Extraction I (µg/g)	Extraction II (µg/g)	Extraction III (µg/g)
4-Hydroxybenzoic acid	5.41 ± 0.5	1.89 ± 0.2	0.25 ± 0.2
Vanillic acid	11.08 ± 0.9	3.07 ± 1.0	0.31 ± 0.2
Vanillin	±	14.19 ± 4.4	1.1 ± 0.4
p-Coumaric acid	±	3.93 ± 0.9	0.27 ± 0.1
Syringaldehyde	±	2.99 ± 0.6	0.26 ± 0.0
Acetovanillone	3.24 ± 0.2	0.68 ± 0.3	0.1 ± 0.02
Ferulic acid	14.9 ± 1.2	3.9 ± 0.9	0.3 ± 0.1
Total	123.54	30.66	2.58