Evidence-Based Complementary and Alternative Medicine / 2017 / Article / Fig 7

Research Article

The Protective Effect of Salidroside on Hypoxia-Induced Corpus Cavernosum Smooth Muscle Cell Phenotypic Transformation

Figure 7

The effect of salidroside on the expression of corpora cavernosum proteins. (a) α-Smooth muscle actin (SMA) and vimentin were detected by immunohistochemistry. Photos were recorded using phase-contrast microscopy (magnification, ×400). (b) Semiquantitative image analysis of α-SMA and vimentin in different groups. (c) Representative western blot of proteins associated with the corpus cavernosum smooth muscle cell phenotype. Beta-actin was used as the loading control. Desmin signals are in the upper band. (d) Quantification of the western blot data. Salidroside increases the expression of the smooth muscle contractile proteins, desmin, and α-SMA and decreases the expression of the corpora cavernosum smooth muscle synthetic protein, vimentin. Data are expressed as means ± SE. compared to the Sham group; and compared to the BCN group (/group).

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