Protective Effects of Wogonin against Alzheimer’s Disease by Inhibition of Amyloidogenic Pathway

Figure 1

Effects of wogonin on AChE activity, Aβ₄₂ oligomerization, and fibrillization. (a) Tet-On Aβ42-GFP SH-SY5Y cells were treated with various concentrations of wogonin (6.25–200 μM), and cell viability was measured by MTT assay. (b) Tet-On Aβ₄₂-GFP SH-SY5Y cells were induced with 10 μg/mL doxycycline in the absence or presence of wogonin (10 and 25 μM) for five days, and AChE activity was then measured by Acetylcholinesterase Assay Kit. (c) Aβ₄₂ (45 μM) was incubated in the absence or presence of wogonin (1 and 10 μM) at room temperature for 3 days and then performed by dot-blot assay. Aβ₄₂ oligomers were detected by using oligomer A11 polyclonal antibody, and total Αβ₄₂ were performed by using 6E10 antibody. (d) HFIP-treated Aβ₄₂ (20 μM) was incubated in the absence or presence of wogonin (1 and 10 μM) assayed by Thioflavin T (ThT), and the fluorescent intensity (excitation/emission = 440 nm/490 nm) was recorded every 30 min for 16 hours at 37°C. (e) Aβ₄₂ (25 μM) in the absence or presence of wogonin (1 μM or 10 μM) was subjected to photo-induced cross-linking unfolded proteins (PICUP) and SDS-PAGE analysis to examine Aβ oligomerization. , .

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