Research Article
Protective Effects of Wogonin against Alzheimer’s Disease by Inhibition of Amyloidogenic Pathway
Figure 3
Wogonin decreased the expression levels of Bax and cleaved PARP in Tet-On Aβ42-GFP SH-SY5Y cells. (a) Aβ42-GFP SH-SY5Y cells were pretreated with/without 10 μM wogonin for one day, and then cells were induced by 10 μg/mL doxycycline (Dox) for five days. After treating with 10 μM wogonin for one days, 3 × 105 Aβ42-GFP SH-SY5Y cells were induced with 10 μg/mL Dox for five days to analyze the expression levels of Bax and cleaved poly (ADP-ribose) polymerase (PARP) by western blotting. Quantitative analyses of the levels of Bax (b) and cleaved PARP (c) were conducted with ImageJ respectively, and actin was used as a loading control. The results are shown as mean ± SEM, , .
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