Central Administration of 1-Deoxynojirimycin Attenuates Hypothalamic Endoplasmic Reticulum Stress and Regulates Food Intake and Body Weight in Mice with High-Fat Diet-Induced Obesity

Figure 2

Effect of 1-deoxynojirimycin (DNJ) on endoplasmic reticulum (ER) stress and leptin signaling in mouse hypothalamic neuronal GT1-7 cells. (a) GT1-7 cells were treated with tunicamycin (TM; 5 μg/mL) as well as DNJ (10–50 μg/mL) for 6 h, after which ER stress and apoptosis responsive markers were measured. (b) GT1-7 cells were treated with TM (5 μg/mL) as well as DNJ (10–50 μg/mL) for 6 h, after which leptin signaling markers were measured. The results of densitometric analysis are the means ± SDs (). values (; ) indicate statistical significance compared to that of vehicle (1 μL of distilled water; DW). values (; ; ) indicate statistical significance compared to that of TM (5 μg/mL).

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