Boi-ogi-to (TJ-20), a Kampo Formula, Suppresses the Inflammatory Bone Destruction and the Expression of Cytokines in the Synovia of Ankle Joints of Adjuvant Arthritic Rats

Table 2

Effects of TJ-20 on Thl/Th2 balance in the synovia of ankle joint in AA rats.

Time	AA rats			AA rats treated with TJ-20
	CXCR3; (Th1)	CCR4; (Th2)	CXCR3/CCR4; Th1/Th2 ratio	CXCR3; (Th1)	CCR4; (Th2)	CXCR3/CCR4; Th1/Th2 ratio
2 weeks	60 ± 6.5	27 ± 4.3	2.22	46.9 ± 5.2	29.8 ± 4.2	1.57
3 weeks	52 ± 5.7	44 ± 7.2	1.18	43.1 ± 6.1	44.6 ± 3.8	0.97
4 weeks	42 ± 4.8	56 ± 6.7	0.75	38 ± 3.6	61.6 ± 3.1	0.62

By use of doubled-immunofluorescence study, we have determined the percentages of CXCR3/W3/25-double-positive cells and CCR4/W3/25-double-positive cells among total W3/25-positive cells in AA rats and AA rats with TJ-20 administration from day 7 after adjuvant injection. Data are the mean ± SD for 6 rats in each group. Asterisks indicate a statistically significant difference from AA rats (, ).