Naoxintong/PPAR Signaling Inhibits Cardiac Hypertrophy via Activation of Autophagy

Figure 1

NXT promotes autophagy. (a) H9c2 cells were treated with 0.5 μg/mL NXT as indicated time course. Cell lysates were subjected to Western blot. (b) Quantitation of LC3-II/LC3-I levels was shown. Results are expressed as means ± SEM (). (c) H9c2 cells were treated with 0.5 μg/mL NXT as indicated time course. Cell lysates were subjected to Western blot. (d) Quantitation of p62/SQSTM1 levels was shown. Results are expressed as means ± SEM (). (e) H9c2 cells were treated with or without 0.5 μg/mL NXT for 3 h. Immunofluorescence analysis was performed. (f) Quantitation of LC3 puncta was shown. Results are expressed as means ± SEM (). (g) H9c2 cells were treated with or without 0.5 μg/mL NXT for 6 h. Autophagy-associated gene expression was assayed by qPCR. Results are expressed as means ± SEM ().

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