1H NMR-Based Metabonomic Study of Functional Dyspepsia in Stressed Rats Treated with Chinese Medicine Weikangning
Table 2
Quantitative comparison of metabolites identified in urine of Weikangning-treated group (WKN) and model group (MS).
Metabolites
Chemical shift
Integral in WKN group (mean ± S.D.) × 10−2
Integral in MS group (mean ± S.D.) × 10−2
VIP
(P < 0.05)
2-Hydroxyvalerate
0.91 (t)
4.087 ± 1.180
5.443 ± 1.093
(↓)
1.762
0.041
Leucine
0.95 (dd)
5.711 ± 0.857
8.219 ± 1.782
(↓)
1.884
0.001
Alanine
1.48 (d)
3.558 ± 0.345
4.877 ± 0.981
(↓)
1.744
0.003
Adipate
1.555 (m)
5.492 ± 0.653
6.826 ± 1.032
−0.645 (↓)
1.816
0.003
N-Acetylglutamate
2.22 (t)
22.047 ± 7.360
7.318 ± 0.913
0.826 (↑)
2.267
0.000
Glucose
3.525 (dd), 3.82 (ddd, m)
21.412 ± 2.625
15.989 ± 1.042
0.930 (↑)
2.397
0.000
Glycine
3.57 (s)
9.757 ± 0.994
12.134 ± 2.001
(↓)
1.625
0.005
Creatine
3.925 (t)
13.164 ± 1.173
11.213 ± 1.026
0.801 (↑)
1.878
0.001
Hippurate
3.965 (d), 7.555 (t), 7.63 (t), 7.83 (t)
21.440 ± 4.307
15.534 ± 2.023
0.708 (↑)
2.027
0.002
Trigonelline
4.435 (s)
4.575 ± 1.232
3.358 ± 0.785
0.657 (↑)
1.635
0.017
cis-Aconitate
5.69 (s)
2.330 ± 0.932
4.625 ± 2.431
(↓)
1.498
0.017
Phenylalanine
7.42 (t)
4.416 ± 1.583
6.770 ± 2.040
(↓)
1.856
0.010
Formate
8.46 (s)
0.970 ± 0.292
0.387 ± 0.102
0.803 (↑)
2.289
0.000
The relative integrals of metabolites were determined by 1D 1H NMR analysis of urine. The values of the correlation number extracted from the correlation plots of OPLS-DA models of WKN versus MS groups. The P values were obtained from Independent-Samples t-test or -test (shown with ). The P values were calculated by Mann–Whitney U test of nonparametric tests and the related integrals were presented in median ± IQR. The arrows (↑/↓) denote higher/lower amounts relative to MS group.
