1H NMR-Based Metabonomic Study of Functional Dyspepsia in Stressed Rats Treated with Chinese Medicine Weikangning
Table 4
Quantitative comparison of metabolites identified in serum of Weikangning-treated group (WKN) and model group (MS).
Metabolites
Chemical shift
Integral in WKN group (mean ± S.D.) × 10−2
Integral in MS group (mean ± S.D.) × 10−2
VIP
(P < 0.05)
Proline
2.04 (m)
0.300 ± 0.040
0.357 ± 0.069
0.660 (↓)
2.535
0.023
Glutamate
2.105 (m)
0.040 ± 0.005
0.052 ± 0.011
0.656 (↓)
2.175
0.001
3-Hydroxybutyrate
2.355 (m)
0.080 ± 0.013
0.112 ± 0.028
0.730 (↓)
2.320
0.008
Citrate
2.555 (AB)
0.008 ± 0.002
0.012 ± 0.002
0.707 (↓)
2.282
0.001
Methionine
2.645 (t)
0.034 ± 0.006
0.042 ± 0.010
0.739 (↓)
2.430
0.045
Glucose
3.44 (t), 3.5 (t), 3.725 (dd), 3.9 (dd)
0.120 ± 0.013
0.096 ± 0.022
−0.736 (↑)
2.430
0.010
Glycerol
3.67 (ABX)
0.134 ± 0.014
0.165 ± 0.047
0.697 (↓)
2.160
0.041
1-Methylhistidine
7.04 (s)
0.000 ± 0.001
0.003 ± 0.002
0.639 (↓)
2.242
0.007
Phenylalanine
7.32 (m), 7.375 (m)
0.000 ± 0.002
0.004 ± 0.007
0.668 (↓)
2.374
0.002
Formate
8.46 (s)
0.038 ± 0.006
0.058 ± 0.023
0.714 (↓)
2.591
0.001
The relative integrals of metabolites were determined by 1D 1H NMR analysis of urine. The values of the correlation number extracted from the correlation plots of OPLS-DA models of WKN versus MS groups. The P values were obtained from Independent-Samples t-test or -test (shown with ). The P values were calculated by Mann–Whitney U test of nonparametric tests and the related integrals were presented in median ± IQR. The arrows (↑/↓) denote higher/lower amounts relative to MS group.
