In Vitro and In Vivo Studies on Quercus acuta Thunb. (Fagaceae) Extract: Active Constituents, Serum Uric Acid Suppression, and Xanthine Oxidase Inhibitory Activity

Table 1

Analytical conditions of the GC-MS and HPLC methods used in this study.

Parameter	Condition
GC-MS
Column	Agilent HP-5MS fused silica capillary column (30 m × 0.25 mm i.d., 0.25 μm film thickness)
Carrier	Helium
Split	1 : 5
Injection volume	1 μL
MS source	230°C
MS quad	150°C
Analytical temperature		Rate	Value	Hold time
	Initial		65°C	10 min
	Ramp	10°C/min	300°C	22 min
	Total	55.5 min
Thermal aux	300°C
Electron ionization	70 ev
Mass range	50–550 amu
Scan method	Full scan
HPLC
Column	Zorbax extended C18 (C18, 4.6 mm × 150 mm, 5 µm)
Flow rate	1 mL/min
Injection volumn	10 μL
UV detection	270 nm
Run time	30 min
Gradient flow	Time (min)	ACN (v/v%)	0.2% phosphoric acid (v/v%)
	0	15	85
	10	15	85
	15	75	25
	20	75	25
	25	100	0
	26	15	85
	30	15	85