Tongxinluo Regulates Expression of Tight Junction Proteins and Alleviates Endothelial Cell Monolayer Hyperpermeability via ERK-1/2 Signaling Pathway in Oxidized Low-Density Lipoprotein-Induced Human Umbilical Vein Endothelial Cells

Figure 2

TXL improved ox-LDL-induced tight junction proteins expression in ox-LDL-induced HUVEC cells. HUVECs were treated with ox-LDL (120 μg/mL) for 24 h prior to treatment with TXL (200 μg/mL) for 4 h. Western blot analysis of ZO-1, occludin, claudin1, and GAPDH was performed (a). GAPDH served as the loading control. Quantitative analysis was performed by measuring protein expression relative to the control. The data were obtained from three independent experiments (b). Each bar represents the mean (±SD) of three independent experiments. versus control, , and versus ox-LDL group.

(a)

(b)