Huang Gan Formula Eliminates the Oxidative Stress Effects of Advanced Oxidation Protein Products on the Divergent Regulation of the Expression of AGEs Receptors via the JAK2/STAT3 Pathway

Figure 8

AOPPs regulate RAGE and AGE-R1 expression by activating the JAK2/STAT3 pathway in HMCs. This effect is inhibited by HGF. In one group, serum-starved HMCs were treated with AOPPs (200 μg/ml) or HGF (200 μg/ml) without AG-490. Cells were serum-starved for 24 h (for JAK2 detection) or 90 min (for p-JAK2 detection). Control cells were not treated. In the other group, cells were pretreated with 10 uM AG-490 for 12 h before AOPP (200 μg/ml) or HGF (800 μg/ml) treatment in serum-free media for a further 24 h. Western blot analysis of JAK2 and STAT3 (a). Western blot analysis of RAGE and AGE-R1 (b). GAPDH was used as a loading control. Data are representative of independent experiments. versus control (first column). versus control (first column).

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