Table of Contents Author Guidelines Submit a Manuscript
Evidence-Based Complementary and Alternative Medicine
Volume 2017 (2017), Article ID 4540124, 8 pages
Research Article

Study of Guanxinning Injection on Regulatory Mechanism of Bcl-2 and Bax by Liquid Nitrogen Freezing-Mediated Femoral Head Necrosis

1Heilongjiang University of Chinese Medicine, Harbin 150040, China
2The Second Affiliated Hospital of Heilongjiang University of Chinese Medicine, Harbin 150001, China
3Research Unit for Biomedical Engineering in Anesthesia and Intensive Care Medicine, Research Unit for Complementary and Integrative Laser Medicine and TCM Research Center Graz, Medical University of Graz, 8036 Graz, Austria
4Privatclinic Lassnitzhoehe, 8301 Lassnitzhoehe, Austria

Correspondence should be addressed to Xilin Xu and Gerhard Litscher

Received 8 December 2016; Accepted 3 April 2017; Published 4 May 2017

Academic Editor: Nunziatina De Tommasi

Copyright © 2017 Xiaofeng Zhang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The objective of this study was to investigate the impact of femoral head perfusion by traditional Chinese medicine Guanxinning injection promoting blood circulation for removing blood stasis on the expression of Bcl-2 and Bax induced by liquid nitrogen freezing-mediated femoral head necrosis. 90 rabbits were randomized into three groups. Normal control group was not subjected to any medication. Saline and Guanxinning group were perfused with 0.9% saline and Guanxinning injection once every three days through the hip joint, respectively. Six animals in each group were sacrificed at weeks 1, 3, 6, 9, and 12. PCR and Western blot measured the expressions of Bcl-2 and Bax in the femoral head. The bax expression in the Guanxinning group reduced at the third week significantly compared to the normal control group (). The Bcl-2 expression in the Guanxinning group increased substantially at the third week ( or ). Prolonged treatment elevated the expression of Bcl-2 in the Guanxinning group while that of Bax reduced remarkably (). Moreover, the ratio of Bcl-2 to Bax increased gradually in the Guanxinning group with prolonged drug administration. Guanxinning injection can inhibit the cell apoptosis of femoral head necrosis through the treatment by femoral head perfusion.