Anti-Inflammatory Effect of Piper attenuatum Methanol Extract in LPS-Stimulated Inflammatory Responses

Figure 2

Effect of Pa-ME on the transcriptional activation of proinflammatory genes. (a) Levels of iNOS, COX-2, and GAPDH mRNA were determined by semiquantitative PCR. (b) Nuclear levels of NF-κB (p65 and p50), AP-1 (c-Jun and c-Fos), and Lamin A/C in nuclear fractions were determined by nuclear fractionation and immunoblotting analysis.

(a)

(b)