Antiaging and Anxiolytic Effects of Combinatory Formulas Based on Four Medicinal Herbs

Figure 7

Effects on ligand binding to membrane neuroreceptors. Effects of extracts of BYPA (■, black); B (▲, green); Y (▼, light blue); P (♦, dark blue); and A (●, yellow). (a) Binding of [³H]-flunitrazepam yielding IC₅₀ values of  mg/ml,  mg/ml,  mg/ml, and  mg/ml for BYPA, B, Y, and A, respectively, and EC₅₀ of  mg/ml for P. (b) Binding of [³H]-muscimol yielding IC₅₀ values of  mg/ml,  mg/ml,  mg/ml,  mg/ml, and  mg/ml, respectively, for BYPA, B, Y, P, and A and (c) binding of [³H]-spiperone yielding IC₅₀ values of  mg/ml,  mg/ml, and  mg/ml for BYPA, B, and Y, with little inhibition by P or A. Data represent mean ± SEM. Total [³H]-ligand binding recorded in each of panels (a)–(c) represented the sum of specific and nonspecific bindings (see Section 2.13), with specific binding constituting more than 90%, 75%, and 60% of total binding, respectively.

(a)

(b)

(c)