trans-Cinnamaldehyde Inhibits Microglial Activation and Improves Neuronal Survival against Neuroinflammation in BV2 Microglial Cells with Lipopolysaccharide Stimulation

Figure 1

Effects of TCA on NO production and cell viability in LPS-stimulated BV2 cells. (a) BV2 cells were pretreated with varying concentration of TCA for 2 h and then stimulated with 0.1 μg/ml LPS for 24 h. Griess assays were performed to measure the amount of nitrite in media of BV2 cells. (b) The inhibitory effects of TCA on NO production were compared to minocycline (Mino) in LPS-stimulated BV2 cells. Cell viability was determined by MTT assay following treatment with TCA (c) and Mino (d). The results are expressed as the percentage of surviving cells over the untreated Control. Each value indicates the mean ± SEM from three independent experiments. , , and versus untreated Controls; , , and versus LPS alone.

(a)

(b)

(c)

(d)