trans-Cinnamaldehyde Inhibits Microglial Activation and Improves Neuronal Survival against Neuroinflammation in BV2 Microglial Cells with Lipopolysaccharide Stimulation

Figure 5

Inhibitory effects of TCA on NF-κB activation and IκBα phosphorylation in LPS-stimulated BV2 cells. (a) Total nuclear protein was extracted to detect NF-κB expression followed by Western blot using an anti-NF-κBp65 antibody. Quantification of protein band densities was normalized to the corresponding levels of Histone 3. (b) Effects of TCA on LPS-induced NF-κB luciferase activity in BV2 cells. (c) Total cytosolic protein was extracted to detect p-IκBα expression, which were normalized with the levels of total IκBα. Value was expressed as relative changes in comparison to Controls, which was set to 1. Data are the mean ± SEM from three independent experiments. , , and versus untreated Controls; , , and versus LPS alone.

(a)

(b)

(c)