trans-Cinnamaldehyde Inhibits Microglial Activation and Improves Neuronal Survival against Neuroinflammation in BV2 Microglial Cells with Lipopolysaccharide Stimulation

Figure 7

TCA inhibited LPS-induced NO production in BV2 cells and neurotoxicity in microglia/neuron coculture by intercepting the NF-κB signaling pathway. (a) BV2 cells were stimulated with LPS (0.1 μg/ml) in the absence or presence of TCA (10 μM), JSH-23 (25 μM), or TCA + JSH-23 for 24 h followed by Griess assay to measure the level of nitrite in medium. (b) MTT assay was performed to analyze PC12 cells viability in microglia/neuron coculture with LPS stimulation in the absence or presence of TCA (10 μM), JSH-23 (25 μM), or TCA + JSH-23 for 24 h. Data are the mean ± SEM from three independent experiments. , , and versus untreated Controls; , , and versus LPS alone.

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