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Evidence-Based Complementary and Alternative Medicine
Volume 2017, Article ID 4846876, 9 pages
https://doi.org/10.1155/2017/4846876
Research Article

Qingchang Wenzhong Decoction Attenuates DSS-Induced Colitis in Rats by Reducing Inflammation and Improving Intestinal Barrier Function via Upregulating the MSP/RON Signalling Pathway

1Beijing University of Chinese Medicine, No. 11, North Third Ring East Road, Beijing 100029, China
2Gastroenterology Department, Dongfang Hospital, Beijing University of Chinese Medicine, No. 6, 1st Section, Fangxingyuan, Fangzhuang, Beijing 100078, China
3China-Japan Friendship Hospital, No. 2, Cherry East Road, Beijing 100029, China
4Gastroenterology Department, The First Affiliated Hospital, Shanxi University of Chinese Medicine, No. 2, Weiyang West Road, Xianyang 712000, China
5Gastroenterology Department, The First Affiliated Hospital, Anhui University of Chinese Medicine, No. 117, Mei Shan Road, Hefei 230031, China

Correspondence should be addressed to Rui Shi; moc.361@885iaihs

Received 5 February 2017; Accepted 21 August 2017; Published 12 October 2017

Academic Editor: Steve Harakeh

Copyright © 2017 Tangyou Mao et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Ulcerative colitis (UC) is a chronic, nonspecific, inflammatory disease for which an effective treatment is lacking. Our previous study found that Qingchang Wenzhong Decoction (QCWZD) can significantly improve the clinical symptoms of UC and ameliorate dextran sulphate sodium- (DSS-) induced ulcerative colitis in rats by downregulating the IP10/CXCR3 axis–mediated inflammatory response. The purpose of the present study was to further explore the mechanism of QCWZD for UC in rats models, which were established by 7-day administration of 4.5% dextran sulphate sodium solution. QCWZD was administered daily for 7 days; then we determined the serum macrophage-stimulating protein concentration (MSP) and recepteur d’origine nantais (RON) expression and its downstream proteins (protein kinase B [Akt], phosphorylated [p] Akt, occludin, zona occluden- [ZO-] 1, and claudin-2) in colon tissue using Western blotting and quantitative polymerase chain reaction. In DSS-induced UC, QCWZD significantly alleviated colitis-associated inflammation, upregulated serum MSP expression and RON expression in the colon, reduced the pAkt levels, promoted colonic occluding and ZO-1 expression, and depressed claudin-2 expression. In conclusion, the MSP/RON signalling pathway plays an important role in the pathogenesis of UC by involving the inflammatory response and improving intestinal barrier function. QCWZD appears to attenuate DSS-induced UC in rats by upregulating the MSP/RON signalling pathway.