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Evidence-Based Complementary and Alternative Medicine
Volume 2017, Article ID 4874865, 10 pages
https://doi.org/10.1155/2017/4874865
Research Article

Effects of Achyrocline satureioides Inflorescence Extracts against Pathogenic Intestinal Bacteria: Chemical Characterization, In Vitro Tests, and In Vivo Evaluation

1Departamento de Bioquímica, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil
2Faculdade de Ciências da Saúde, Centro Universitário Ritter dos Reis (UniRitter), Porto Alegre, RS, Brazil
3Instituto de Ciência e Tecnologia de Alimentos, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil
4Instituto de Tecnologia do Petróleo, Pontíficia Universidade Católica do Rio Grande do Sul (PUCRS), Porto Alegre, RS, Brazil
5Instituto de Quimica, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil
6Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil

Correspondence should be addressed to Karla Suzana Moresco; moc.liamg@ocseromalrak

Received 6 May 2017; Revised 23 June 2017; Accepted 17 July 2017; Published 7 September 2017

Academic Editor: Daniela Rigano

Copyright © 2017 Karla Suzana Moresco et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Three Achyrocline satureioides (AS) inflorescences extracts were characterized: (i) a freeze-dried extract prepared from the aqueous extractive solution and (ii) a freeze-dried and (iii) a spray-dried extract prepared from hydroethanol extractive solution (80% ethanol). The chemical profile, antioxidant potential, and antimicrobial activity against intestinal pathogenic bacteria of AS extracts were evaluated. In vitro antioxidant activity was determined by the total reactive antioxidant potential (TRAP) assay. In vivo analysis and characterization of intestinal microbiota were performed in male Wistar rats (saline versus treated animals with AS dried extracts) by high-throughput sequencing analysis: metabarcoding. Antimicrobial activity was tested in vitro by the disc diffusion tests. Moisture content of the extracts ranged from 10 to 15% and 5.7 to 17 mg kg−1 of fluorine. AS exhibited antioxidant activity, especially in its freeze-dried form which also exhibited a wide spectrum of antimicrobial activity against intestinal pathogenic bacteria greater than those observed by the antibiotic, amoxicillin, when tested against Bacillus cereus and Staphylococcus aureus. Antioxidant and antimicrobial activities of AS extracts seemed to be positively correlated with the present amount of flavonoids. These findings suggest a potential use of AS as a coadjuvant agent for treating bacterial-induced intestinal diseases with high rates of antibiotic resistance.