Table of Contents Author Guidelines Submit a Manuscript
Evidence-Based Complementary and Alternative Medicine
Volume 2017, Article ID 5326909, 8 pages
https://doi.org/10.1155/2017/5326909
Research Article

Genome-Wide Profiling Reveals That Herbal Medicine Jinfukang-Induced Polyadenylation Alteration Is Involved in Anti-Lung Cancer Activity

1Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, College of Life Sciences, Northwest University, 229 Taibai Road, Xi’an 710069, China
2Shanghai Center for Systems Biomedicine, School of Biomedical Engineering, State Key Laboratory on Oncogene and Bio-ID Center, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, China

Correspondence should be addressed to Jing Tian; nc.ude.uwn@gnijnait

Received 6 April 2017; Revised 15 June 2017; Accepted 13 September 2017; Published 1 November 2017

Academic Editor: Oliver Micke

Copyright © 2017 Yao Kou et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Alternative polyadenylation (APA) plays an important role in regulation of genes expression and is involved in many biological processes. As eukaryotic cells receive a variety of external signals, genes produce diverse transcriptional isoforms and exhibit different translation efficiency. The traditional Chinese medicine (TCM) Jinfukang (JFK) has been effectively used for lung cancer treatment. In this study, we investigated whether JFK exerts its antitumor effect by modulating APA patterns in lung cancer cells. We performed a genome-wide APA site profiling analysis in JFK treated lung cancer cells A549 with 3T-seq approach that we reported previously. Comparing with those in untreated A549, in JFK treated A549 we observed APA-mediated 3′ UTRs alterations in 310 genes including 77 genes with shortened 3′ UTRs. In particular, we identified TMEM123, a gene involved in oncotic cell death, which produced transcripts with shortened 3′ UTR and thus was upregulated upon JFK treatment. Taken together, our studies suggest that APA might be one of the antitumor mechanisms of JFK and provide a new insight for the understanding of TCM against cancer.