Anticancer Effects of Salvia miltiorrhiza Alcohol Extract on Oral Squamous Carcinoma Cells

Figure 3

Danshen alcohol extract induces caspase-3 apoptotic pathway in HSC-3 cells. (a) Cells were treated with indicated concentrations of Danshen alcohol extract. Apoptosis was estimated using ITC Active Caspase-3 Apoptosis Assays. DMSO and camptothecin (Camp) served as a control and positive control, respectively, for caspase-3 activity. (b) Cells were incubated with different concentrations of Danshen alcohol extract. Mitochondrial membrane potential (ΔΨm) was measured by flow cytometry with JC-1 staining. DMSO and carbonyl cyanide 3-chlorophenylhydrazone (CCCP) served as a control and positive control, respectively.

(a)

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