Salidroside Protects against MPP+-Induced Neuronal Injury through DJ-1-Nrf2 Antioxidant Pathway

Figure 7

Role of Nrf2 in Sal-induced effects on key antioxidant regulator expression in MPP+-treated SH-SY5Y cells. H-SY5Y cells were transfected with siDJ-1, pretreated with 25–100 μM Sal for 24 h, and then exposed to 500 μM MPP+ for an additional 24 h. ((a) and (b)) mRNA expression of DJ-1, Nrf2, GCLc, SOD1, and SOD2 was determined and statistical analysis was showed. ((c) and (d)) Protein expression of DJ-1, Nrf2, GCLc, SOD1, and SOD2 was determined and statistical analysis was showed. (e) ROS level was determined using DCFH-DA staining. (f) GSH level was determined by an assay kit. , compared with control. , compared with MPP⁺. , compared with Sal.

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