Dangguijihwang-tang and Dangguijakyak-san Prevent Menopausal Symptoms and Dangguijihwang-tang Prevents Articular Cartilage Deterioration in Ovariectomized Obese Rats with Monoiodoacetate-Induced Osteoarthritis

Figure 3

Changes of serum glucose and insulin levels during oral glucose tolerance test at the end of experiment. Ovariectomized (OVX) rats were provided with a 45% fat diet containing (1) 1% lyophilized DJY powder + MIA injection (DJY), (2) 1% lyophilized DJH powder + MIA injection (DJH), (3) 30 μg/kg body weight 17β-estradiol + 1% dextrose + MIA injection (positive control), (4) 1% dextrose + MIA injection (placebo, control), or (5) 1% dextrose with no MIA injection (normal control). After 4 weeks of the assigned diets, an articular injection of MIA into the right knee was made in all OVX groups except the normal-control group and the assigned diets were provided for an additional 3 weeks. At 7th week rats were orally given 2 g glucose/kg body weight and serum glucose and insulin levels were measured. (a) Changes of serum glucose levels. (b) Changes of serum insulin levels. (c) Area under the curve of serum glucose and insulin levels. Each bar and error bar represents the mean ± SD from 10 rats per group. Different letters indicate significant differences in the treatment groups of OVX rats at each time point identified by Tukey’s test at .

(a)

(b)

(c)