Dangguijihwang-tang and Dangguijakyak-san Prevent Menopausal Symptoms and Dangguijihwang-tang Prevents Articular Cartilage Deterioration in Ovariectomized Obese Rats with Monoiodoacetate-Induced Osteoarthritis

Figure 7

Histopathological features of osteoarthritic lesions in the knee joints of rats at 21 days after intra-articular injection of monoiodoacetate (MIA). Ovariectomized (OVX) rats were provided with a 45% fat diet containing (1) 1% lyophilized DJY powder + MIA injection (DJY), (2) 1% lyophilized DJH powder + MIA injection (DJH), (3) 30 μg/kg body weight 17β-estradiol + 1% dextrose + MIA injection (positive control), (4) 1% dextrose + MIA injection (placebo, control), or (5) 1% dextrose with no MIA injection (normal control). After 4 weeks of the assigned diets, an articular injection of MIA into the right knee was made in all OVX groups except the normal-control group and the assigned diets were provided for an additional 3 weeks. (a) Images of H-E staining in the right knee (×10). (b) Quantification of the joint space and structural damage. (c) Safranin O-fast green staining in the right knee (×10). (d) Quantification of cartilage damage. Each bar and error bar represents the mean ± SD from 4 rats per group. Different letters indicate significant differences in the treatment groups of OVX rats at each time point identified by Tukey’s test at .

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