Parasite Killing of Leishmania (V) braziliensis by Standardized Propolis Extracts

Figure 3

L. (V) braziliensis promastigote viability assessed by fluorescence microscopy. Live cells were stained with Acridine Orange (green color) and dying cells with Ethidium Bromide (red color). L. braziliensis promastigotes were treated with medium alone or with dry, alcoholic, or glycolic extracts of propolis (50 μg/mL) for 96 h. Amphotericin B treatment (0.5 μg/mL) for 24 h was used as a positive control for cell death. Magnification: 1000x.