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Evidence-Based Complementary and Alternative Medicine
Volume 2017, Article ID 6072573, 12 pages
Research Article

Barley Seedling Extracts Inhibit RANKL-Induced Differentiation, Fusion, and Maturation of Osteoclasts in the Early-to-Late Stages of Osteoclastogenesis

1Division of Crop Foundation, National Institute of Crop Science (NICS), Rural Development Administration (RDA), Jeonbuk 565-851, Republic of Korea
2Department of Biological Sciences, College of Natural Science, Chonbuk National University, Jeonbuk 561-756, Republic of Korea
3College of Crop Science and Biotechnology, Dankook University, Cheonan 330-714, Republic of Korea
4Division of Research Development and Education, National Institute of Chemical Safety, Ministry of Environment, Daejeon 305-343, Republic of Korea

Correspondence should be addressed to Woo Duck Seo; rk.aerok@2002dws

Received 7 March 2017; Revised 3 April 2017; Accepted 18 April 2017; Published 8 May 2017

Academic Editor: Vishal Chandra

Copyright © 2017 Sik-Won Choi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The number of patients with osteoporosis is increasing worldwide, and a decrease in bone mass is a main risk factor for fracture. The prevention of bone loss is critical for improving the quality of life for patients. However, the long-term use of antiosteoporotic agents is limited due to their side effects. Barley has been traditionally ingested for thousands of years as a safe, natural food with pharmaceutical properties, and its seedling can enhance the biological activity of the medicinal components found in food. This study aimed to clarify the antiresorptive activity of barley seedling and its mode of action. Barley seedling extracts (BSE) dose-dependently inhibited RANKL-induced osteoclast differentiation with alteration of IκB degradation, c-Fos, and NFATc1 molecules in the early-to-middle stages of osteoclastogenesis. In the late phase of osteoclastogenesis, BSE also prevented DC-STAMP and cathepsin K, which are required for cell fusion and bone degradation, such as osteoclast function. In conclusion, barley seedling from natural foods may provide long-term safety and be useful for the prevention or treatment of osteoclast-mediated bone metabolic diseases, including osteoporosis.