Barley Seedling Extracts Inhibit RANKL-Induced Differentiation, Fusion, and Maturation of Osteoclasts in the Early-to-Late Stages of Osteoclastogenesis

Figure 2

BSE inhibits the RANKL-mediated expression level of c-Fos/NFATc1. (a) The BMMs were stimulated with RANKL (10 ng/ml) and M-CSF (30 ng/ml) in the presence or absence of BSE (3 μg/ml) for the indicated times. Total RNA was then isolated using TRIzol reagent, and the mRNA expression levels were evaluated using real-time PCR. GAPDH was used as the internal control. ; (versus the vehicle control). (b) The effect of BSE on the protein expression level of RANKL-induced transcription factors was evaluated using Western blot analysis. Actin was used as the internal control. Data are representative of at least three experiments.

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