Spatholobus suberectus Exhibits Antidiabetic Activity In Vitro and In Vivo through Activation of AKT-AMPK Pathway

Figure 3

Cell viability and glucose uptake associated with signaling activity of Spatholobus suberectus in C2C12 cells. C2C12 cells were seeded at a density of 2 × 10⁵ cells per well (96-well plate), and then the MTT assay (a) was performed. Differentiated C2C12 cells were incubated with AeSs and EeSs with or without insulin at indicated concentration for 24 hr and the glucose uptake (b) was measured as described in Materials and Methods. (c) Differentiated C2C12 cells were collected and mRNA levels of genes were determined by RT-PCR; (d and e) protein extracts were prepared and subjected to western blot assay using indicated primary antibody; beta-actin levels were used as a control for equal loading (d and e). versus control; versus control using Student’s t-test. NT: not treated; Ros: rosiglitazone-treated; AeSs: aqueous extract of S. suberectus; EeSs: ethanolic extract of S. suberectus.

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