Ping-Chong-Jiang-Ni Formula Induces Apoptosis and Inhibits Proliferation of Human Ectopic Endometrial Stromal Cells in Endometriosis via the Activation of JNK Signaling Pathway

Figure 3

PCJNF induced the activation of JNK signaling pathway in EESCs. EESCs were exposed to different concentration of PCJNF-containing serum (5%, 10%, 20%, and 40%). Representative Western blot of beta-actin, total JNK, and phospho-JNK was shown. Different concentration of PCJNF-containing serum increased the expression of p-JNK but not the total JNK, indicating the activation of JNK signaling pathway. Compared with CONT group, 5% and 10% PCJNF-containing serum had certain effect on the activation of JNK pathway, while 20% and 40% PCJNF-containing serum resulted in obvious activation of JNK pathway. The experiment was performed in triplicate. CONT: complete medium.