Ping-Chong-Jiang-Ni Formula Induces Apoptosis and Inhibits Proliferation of Human Ectopic Endometrial Stromal Cells in Endometriosis via the Activation of JNK Signaling Pathway

Figure 4

The suppressive effect of PCJNF-containing serum was mediated by JNK signaling pathway in EESCs. (a) EESCs were treated with 20% PCJNF-containing serum and different controls for 0, 24, 48, 72, and 96 h, respectively. The cell viability was assessed by CCK8 assay. Compared with different control groups (CONT, DMSO, and NS), 20% PCJNF-containing serum had significant suppressed effect on cell viability after 24 h, while the JNK inhibitor SP600125 attenuated this effect only after 48 h. The experiment was performed in triplicate. (b) Meanwhile, JNK inhibitor SP600125 deactivates the JNK signaling pathway in EESCs, when compared with different controls (CONT, DMSO, and NS). While there was no significant difference of cell proliferation in EESCs between the “NS” and “NS + SP600125” group (). (c) The total and phosphorylated ERK and p38 of EESCs did not change markedly, when compared with different controls. The experiment was performed in triplicate. NS: rat serum from saline treatment; PCJNF: rat serum with PCJNF treatment.

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