Optimization of Extraction Process for Antidiabetic and Antioxidant Activities of Kursi Wufarikun Ziyabit Using Response Surface Methodology and Quantitative Analysis of Main Components
Table 2
BBD and the observed responses for the independent variables of extraction conditions.
Run
Independent variables
Responses
1
50 (0)
80 (1)
20 (0)
4 (1)
35.88
45.24
79.24
77.54
69.14
2
50 (0)
80 (1)
10 (−1)
3 (0)
34.02
43.07
85.49
87.71
57.76
3
50 (0)
80 (1)
20 (0)
2 (−1)
38.58
47.4
67.66
77.81
68.52
4
50 (0)
60 (1)
20 (0)
2 (−1)
33.8
47.33
71.93
87.45
67.17
5
30 (−1)
70 (0)
20 (0)
4 (1)
35.22
43.53
80.34
98.43
67.54
6
70 (1)
80 (1)
20 (0)
3 (0)
35.75
47.05
82.62
98.17
76.91
7
50 (0)
70 (0)
20 (0)
3 (0)
39.14
47.08
87.93
94.12
75.58
8
70 (1)
70 (0)
20 (0)
2 (−1)
32.88
47.36
69.25
98.10
71.22
9
30 (−1)
70 (0)
30 (1)
3 (0)
37.96
46.01
72.72
93.77
66.06
10
50 (0)
70 (0)
30 (1)
2 (−1)
40.74
45.89
75.41
83.32
67.78
11
50 (0)
60 (1)
30 (1)
3 (0)
33.5
48.05
81.25
94.12
55.17
12
30 (−1)
70 (0)
20 (0)
2 (−1)
38.71
46.96
67.13
81.41
77.12
13
50 (0)
70 (0)
20 (0)
3 (0)
39.27
48.65
87.28
98.25
77.72
14
50 (0)
80 (1)
30 (1)
3 (0)
39.13
41.76
82.16
91.69
67.75
15
70 (1)
60 (1)
20 (0)
3 (0)
33.41
47.73
73.82
78.36
55.70
16
30 (−1)
70 (0)
10 (−1)
3 (0)
27.88
35.99
85.25
98.71
64.39
17
70 (1)
70 (0)
30 (1)
3 (0)
35.45
41.21
74.28
98.65
82.37
18
50 (0)
70 (0)
10 (−1)
2 (−1)
30.88
35.84
68.02
97.72
72.73
19
70 (1)
70 (0)
20 (0)
4 (1)
34.49
44.47
71.83
88.23
77.84
20
50 (0)
70 (0)
20 (0)
3 (0)
38.03
47.99
88.05
98.02
75.03
21
30 (−1)
60 (1)
20 (0)
3 (0)
34.57
48.23
78.41
98.69
65.58
22
50 (0)
60 (1)
10 (−1)
3 (0)
29.83
37.74
78.82
91.81
51.10
23
50 (0)
60 (1)
20 (0)
4 (1)
36.6
45.53
66.04
93.98
55.45
24
50 (0)
70 (0)
20 (0)
3 (0)
38.88
48.04
86.05
96.89
77.13
25
50 (0)
70 (0)
10 (−1)
4 (1)
30.24
40.41
83.20
94.92
57.53
26
50 (0)
70 (0)
30 (1)
4 (1)
40.35
36.95
67.15
97.40
79.90
27
30 (−1)
80 (1)
20 (0)
3 (0)
36.15
45.05
87.74
75.61
52.48
28
50 (0)
70 (0)
20 (0)
3 (0)
39.37
46.7
81.27
95.87
74.11
29
70 (1)
70 (0)
10 (−1)
3 (0)
29.27
41.22
79.06
97.58
59.85
, ethanol concentration (%); , temperature (°C); , Sample-to-solvent ratio (w/v); , extraction time (h); , yield of extraction (%); , total polyphenol content (%); , PTP-1B inhibition rate (the sample concentration is 5 μg/mL, %); , α-glucosidase inhibition rate (the sample concentration is 50 μg/mL, %); , ABTS inhibition rate (the sample concentration is 8 μg/mL, %).
