Induction of Cell Cycle Arrest and Apoptotic Response of Head and Neck Squamous Carcinoma Cells (Detroit 562) by Caffeic Acid and Caffeic Acid Phenethyl Ester Derivative

Figure 4

Flow cytometric analysis demonstrated a significant increase in proportion of total apoptotic cells in the NHSCC cells following exposure to mainly CAPE 100 μM and CA 100 μM, also with increased percentage of dead cells. Total apoptotic cells significantly increased following exposure to 100 μM CAPE, compared with 100 μM CA after 48 hours (). Specifically, the difference between CA and CAPE for both concentrations 50 μM and 100 μM in the percentage of early apoptotic cells was slight for the time laps 24 h, whereas the difference between them in the percentage of late apoptotic cells was significant for concentration 100 μM (). Vertical bars represent the standard deviation of means (SD) ( experiments). value