Comparative Phytochemical Profiling and In Vitro Antioxidant Activity of Extracts from Raw Materials, Tissue-Cultured Plants, and Callus of Oroxylum indicum (L.) Vent.

Figure 2

TLC chromatogram of Oroxylum indicum extracts; 1 = OIFL1, 2 = OIFL2, 3 = OIFL3, 4 = OIFL4, 5 = OIFL4, 6 = OIS, 7 = OIPC, 8 = baicalin, 9 = baicalein, 10 = chrysin, 11 = chlorogenic acid, 12 = OIL, 13 = OIWF, 14 = OICB, 15 = OICG, 16 = OICW, 17 = OICY, 18 = OIMS, 19 = OITPd3, 20 = OITPd5, 21 = OITPd7, 22 = OITPw2, 23 = OITPw3, 24 = OITPw4, 25 = baicalin, 26 = baicalein, 27 = chrysin, 28 = chlorogenic acid, 29 = OITPw5R, 30 = OITPw5S, 31 = OITPw6R, 32 = OITPw6S, 33 = OITPw7R, 34 = OITPw7S, 35 = OITPw8R, and 36 = OITPw8S, adsorbent: silica gel GF254. Solvent system: A = hexane-ethyl acetate-acetic acid-formic acid (5 : 5 : 1 : 1 v/v/v/v), B = toluene-ethyl acetate-formic acid (25 : 25 : 7.5 v/v/v), detection: 1 = UV 254 nm, 2 = UV 366 nm, and 3 = NP/PEG under UV 366 nm. Band identification system A: baicalin (Rf = 0.06), chlorogenic acid (Rf = 0.11), baicalein (Rf = 0.64), and chrysin (Rf = 0.71). Band identification system B: baicalin (Rf = 0.02), chlorogenic acid (Rf = 0.11), baicalein (Rf = 0.60), and chrysin (Rf = 0.70).

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