Standardized Kaempferia parviflora Extract Inhibits Intrinsic Aging Process in Human Dermal Fibroblasts and Hairless Mice by Inhibiting Cellular Senescence and Mitochondrial Dysfunction

Figure 1

Effect of KPE on cell growth promotion and SA-β-gal activity. Hs68 cells were pretreated with KPE (1–10 μg/ml) or resveratrol (10 μM) for 24 h. After 2 h of H₂O₂ exposure, the cells were cultured for an additional 72 h. (a) The number of proliferating cells was measured using an MTT assay. (b) The cellular SA-β-gal activity was detected using a fluorescence reader at 365 nm excitation and 460 nm emission. Data are represented as mean ± SD from triplicate independent experiments. ^##p < 0.01 (normal versus H₂O₂ control); < 0.05 and < 0.01 (H₂O₂ control versus sample-treated cells).

(a)

(b)