Cartilage Protection and Analgesic Activity of a Botanical Composition Comprised of Morus alba, Scutellaria baicalensis, and Acacia catechu

Table 2

Unexpected synergistic effect of UP1306 and UP446 combination in reducing pain sensitivity.

Pain inhibition synergy calculation (%)
Weeks after MIA		Wk 1	Wk 2	Wk 3	Wk 4	Wk 5
UP446 ()	250 mg/kg	28.9	28.1	34.7	37.6	40.8
UP1306 ()	400 mg/kg	17.1	23.6	30.1	32.1	33.8
Equation	()	46.0	51.7	64.8	69.7	74.6
		4.9	6.6	10.4	12.1	13.8
Expected		41.1	45.1	54.4	57.6	60.8
Observed	Composition^†; (650 mg/kg)	47.9	53.8	64.4	60.7	62.5

(i) ^†Composition: UP1306 + UP446; (ii) UP446 (); UP1306 (); Colby’s equation: []. Data interpretation: a formulation of two or more materials together will presume to have unexpected synergy when the observed value of a certain end point measurement is greater than or equal to the hypothetically calculated values. That is, observed ≥ expected = synergy. Observed = composition pain inhibition; expected = theoretical calculated pain inhibition value.