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Evidence-Based Complementary and Alternative Medicine
Volume 2017 (2017), Article ID 7209851, 10 pages
https://doi.org/10.1155/2017/7209851
Research Article

Major Phytochemical as γ-Sitosterol Disclosing and Toxicity Testing in Lagerstroemia Species

1Department of Biology, Faculty of Science, Khon Kaen University, Khon Kaen, Thailand
2Genetics and Environmental Toxicology Research Group, Khon Kaen University, Khon Kaen, Thailand
3Faculty of Environment and Resource Studies, Mahasarakham University, Maha Sarakham, Thailand
4Faculty of Law, Khon Kaen University, Khon Kaen, Thailand

Correspondence should be addressed to Arunrat Chaveerach; ht.ca.ukk@ahccar

Received 20 September 2016; Revised 16 November 2016; Accepted 20 December 2016; Published 16 January 2017

Academic Editor: Ciara Hughes

Copyright © 2017 Prapaparn Sirikhansaeng et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Medicinal plants in genus Lagerstroemia were investigated for phytochemical contents by GC-MS and HPLC with ethanol and hexane extracts and their toxicity MTT and comet assay on human peripheral blood mononuclear cells (PBMCs). γ-Sitosterol is the major component found in all species at 14.70–34.44%. All of the extracts, except for L. speciosa ethanol extract, showed high percentages of cell viability. The IC50 value, 0.24 mg/mL, of ethanol L. speciosa extract predicted an LD50 of 811.78 mg/kg, which belongs to WHO Class III of toxic chemicals. However, in-depth toxicity evaluation by the comet assay showed that the four tested species induced significant () DNA damage in PBMCs. γ-Sitosterol was previously reported to possess antihyperglycemic activity by increasing insulin secretion in response to glucose. Nonetheless, consumers should consider its toxicity, and the amount of consumption should be of concern.