Research Article

Potential Antitumor Activity and Apoptosis Induction of Glossostemon bruguieri Root Extract against Hepatocellular Carcinoma Cells

Figure 3

MRE induced apoptosis in HepG2 and Hep3B cells in a caspase-dependent manner. Cells were either untreated or treated with MRE at different concentrations (1/10 and 1/2 IC50) for 48 hrs. Lysed cells were subjected to (a) enzyme linked immunosorbent apoptosis assay to measure the histone release as an indication for apoptosis and (b) caspase-3 activity assay. Each assay was done in triplicate and standard deviation was calculated. Data are presented as mean ± SD. P value was calculated versus control cells: and .
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