Methanol Extract of Holarrhena antidysenterica Inhibits the Growth of Human Oral Squamous Cell Carcinoma Cells and Osteoclastogenesis of Bone Marrow Macrophages

Figure 4

The inhibitory effect of HABE on RANKL-induced osteoclastogenesis. (a) BMMs (5 × 10⁴ cells/well) were treated with the indicated HABE concentrations for 5 days in α-MEM containing 10% FBS and M-CSF (30 ng/ml). The cell viability was determined using an MTT assay. (b) BMMs (5 × 10⁴ cells/well) were incubated with HABE at the indicated concentrations in the presence of 10% FBS, M-CSF (30 ng/ml), and RANKL (100 ng/ml) for 5 days. TRAP-positive multinucleated osteoclasts with more than three nuclei were counted. Data are expressed as the mean ± SE. versus BMMs treated with M-CSF alone. versus BMMs treated with M-CSF and RANKL.

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