Rosemary Extracts Upregulate Nrf2, Sestrin2, and MRP2 Protein Level in Human Hepatoma HepG2 Cells

Figure 3

Rosemary extract (RE), carnosic acid (CA), and carnosol (CL) increased the protein levels of Nrf2, Sestrin2, and MRP2 in HepG2 cells. HepG2 cells (1 × 10⁶ cells per well) were treated with RE (0, 30, 50, and 100 μg/mL) or CA and CL (0, 30, 50, and 100 μM), respectively, for 24 hrs. (a) Western Blot analysis of the protein level of Nrf2, MRP2, and Sestrin2 in RE treated HepG2 cells. Left column: protein level in RE treated HepG2 cells after 24 hrs; right column: densitometric analysis of RE Western Blot results. (b) Western Blot analysis of the protein level of Nrf2, MRP2, and Sestrin2 in CA treated HepG2 cells. Left column: protein level in CA treated HepG2 cells after 24 hrs; right column: densitometric analysis of CA Western Blot results. (c) Western Blot analysis of the protein level of Nrf2, MRP2, and Sestrin2 in CL treated HepG2 cells. Left column: protein level in CL treated HepG2 cells after 24 hrs; right column: densitometric analysis of CL Western Blot results.

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