Kyungheechunggan-Tang-01, a New Herbal Medication, Suppresses LPS-Induced Inflammatory Responses through JAK/STAT Signaling Pathway in RAW 264.7 Macrophages

Figure 3

KCT-01, KCT-02, or IJCGT inhibited production of NO and PGE₂ in LPS-induced RAW264.7 macrophages at noncytotoxic concentrations. Cells were treated with KCT-01, KCT-02, or IJCGT at various concentrations (6.25–100 μg/mL) plus LPS (1 μg/mL) or LPS alone for 24 h. (a) Cell viability was measured by MTT assay and NO production was measured using Griess reaction assay. (b) PGE₂ production was measured using an EIA kit. Values are expressed as means ± SD of three independent experiments. versus control, and versus LPS-treated cells.

(a)

(b)

(c)