Supercritical Fluid Extraction of Pyrrolidine Alkaloid from Leaves of Piper amalago L.

Figure 1

(a) Chromatogram of an extract obtained by supercritical CO₂ + 5% methanol (v : v) at 40°C, 200 bar for 60 min. (b) Chromatogram of the examined alkaloid whose retention time was 4 min. Chromatograph conditions: column Kinetex C18 column Phenomenex (150 4.6 mm, d.i.), at 25°C; mobile phase used was 58% of acetonitrile and 42% of water containing 1% of acetic acid, changing to 100% of acetonitrile at 7 min to 11 min, returning to 58% of acetonitrile and 42% of water at 11 min to 12 min, flow rate of 1 ml/min; temperature at 25°C; detection at 260 nm.

(a)

(b)