Supercritical Fluid Extraction of Pyrrolidine Alkaloid from Leaves of Piper amalago L.

Table 1

Orthogonal array design matrix OAD OA₉(3)⁴ and experimental results of alkaloid 1 yield.

Run	Factors				Responses
	Cosolvent	(min)	(°C)	(bar)	(mg g^−1) ()
1	Ethanol	20	40	150	0.29 0.02
2	Ethanol	40	50	200	1.2 0.3
3	Ethanol	60	60	250	1.59 0.07
4	Methanol	20	50	250	1.0 0.3
5	Methanol	40	60	150	0.7 0.9
6	Methanol	60	40	200	3.8 0.8
7	Propyleneglycol	20	60	200	1.9 0.3
8	Propyleneglycol	40	40	250	1.86 0.08
9	Propyleneglycol	60	50	150	0.73 0.01

= yield of the alkaloid in the plant.
Results are expressed as mean standard deviation ().