Supercritical Fluid Extraction of Pyrrolidine Alkaloid from Leaves of Piper amalago L.

Table 2

Analysis of variance (ANOVA) of four parameters for the extraction using supercritical CO₂ and cosolvents.

	Sum of squares	Degrees of freedom	-ratio		value	Effect
Cosolvent	2.3	2	1.1	7.4	0.01	Significant
Time	3.1	2	1.5	10.0	0.005	Significant
Temperature	3.1	2	1.6	10.0	0.005	Significant
Pressure	8.3	2	4.1	26.6	0.0002	Significant
Residual	1.4	9	0.15