Supercritical Fluid Extraction of Pyrrolidine Alkaloid from Leaves of Piper amalago L.

Table 3

Alkaloid yield for extraction without cosolvents (only CO₂) at different pressures and temperatures ( min).

Run	Factors		Responses
	(°C)	(bar)	(mg g^−1) ± (%)
10	40	150	0.42 ± 1
11	50	150	0.54 ± 2
12	60	150	0.48 ± 2
13	40	200	0.97 ± 3
14	50	200	0.90 ± 12
15	60	200	1.04 ± 2
16	40	250	0.75 ± 2
17	50	250	0.71 ± 1
18	60	250	1.11 ± 2

= yield of the alkaloid in the extract. = yield of the alkaloid in the plant.
Results are expressed as mean relative standard deviation ().