5,7-Dihydroxyflavone Analogues May Regulate Lipopolysaccharide-Induced Inflammatory Responses by Suppressing IκBα-Linked Akt and ERK5 Phosphorylation in RAW 264.7 Macrophages

Figure 3

Effects of 1, 9, and inhibitors on expression levels of iNOS, COX1, COX2, and inflammation-related proteins in RAW 264.7 cells. RAW 264.7 cells were seeded into a 6-well plate ( cells/well) and incubated for 2 hours. Then they were stimulated with LPS of 100 ng/well with or without various concentrations of a dimethyl sulfoxide (DMSO) solution of a 5,7-dihydroxyflavone analogue or an inhibitor for 16 hours. Detailed procedures for the protein collection/evaluation are described in the text. Same cell lysate was used for the assessment of six proteins and GAPDH was used as the standard for quantification of all proteins. Labels present the compound names and concentrations (μM). Data are expressed as mean ± SD from 3 independent experiments. Control group were presented by the open circles. : and : .