Active Ingredients of Epimedii Folium and Ligustri Lucidi Fructus Balanced GR/HSP90 to Improve the Sensitivity of Asthmatic Rats to Budesonide

Figure 3

Effects of Bun and EL on GRα. GRα protein and mRNA expressions in lung tissues were detected by IHC, WB, and qPCR. (a) Summarized data of positive area of GRα protein expression in lung tissues measured by IHC. (b) Summarized data of IOD of GRα protein expression in lung tissues measured by IHC. (c) Summarized data of sumdensity of GRα protein expression in lung tissues measured by WB, normalized to β-actin. (d) The mRNA expression of GRα was measured by qPCR analysis with 18S as an internal control. (e) Representative IHC photographs of GRα were viewed at a magnification of 400x in control, asthma, Bun, EL, and Bun&EL groups. (f) Representative WB photographs of GRα were viewed, and β-actin was used for normalization in control, asthma, Bun, EL, and Bun&EL groups. Values are expressed as mean ± SD. (a, b), (c), or (d). and versus control group; versus asthma group; and versus Bun group. .

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