Active Ingredients of Epimedii Folium and Ligustri Lucidi Fructus Balanced GR/HSP90 to Improve the Sensitivity of Asthmatic Rats to Budesonide

Figure 4

Effects of Bun and EL on GRβ expression. GRβ protein and mRNA expressions in lung tissues were detected by IHC, WB, and qPCR. (a) Summarized data of positive area of GRβ protein expression in lung tissues measured by IHC. (b) Summarized data of IOD of GRβ protein expression in lung tissues measured by IHC. (c) Summarized data of sumdensity of GRβ protein expression in lung tissues measured by WB, normalized to β-actin. (d) The mRNA expression of GRβ was measured by qPCR analysis with 18S as an internal control. (e) Representative IHC photographs of GRβ were viewed at a magnification of 400x in control, asthma, Bun, EL, and Bun&EL groups. (f) Representative WB photographs of GRβ were viewed, and β-actin was used for normalization in control, asthma, Bun, EL, and Bun&EL groups. Values are expressed as mean ± SD. (a, b), (c), and (d). and versus control group; versus asthma group; versus Bun group.

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