Research Article

Cardioprotective Effect of Danshensu against Ischemic/Reperfusion Injury via c-Subunit of ATP Synthase Inhibition

Figure 3

Cardiac apoptosis rate assay. (a) TUNEL detection. Nuclear was stained by DAPI and presents blue fluorescent in heart tissue. Apoptosis was detected by DNA strand breaks stained by TUNEL kit and present green fluorescent. Scale bar = 100 um. (b) Apoptosis rate was calculated by IPP software from five visual fields in each group. Apoptosis rate declined significantly in DSS group. (c) Western blot results of caspase-3 protein expression. (d) Statistic data of caspase-3 protein expression. Values were expressed as mean ± SEM. , , compared to control group. , , compared to model group.
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