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Evidence-Based Complementary and Alternative Medicine
Volume 2017 (2017), Article ID 8256278, 9 pages
https://doi.org/10.1155/2017/8256278
Research Article

Renoprotective Effects of Total Glucosides from Paeony against Nephrotoxicity Induced by Total Alkaloids from Semen Strychni

1School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China
2School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China

Correspondence should be addressed to Xiaohui Chen; moc.liamtoh@uhpys_hxc

Received 1 May 2017; Revised 18 July 2017; Accepted 2 August 2017; Published 22 October 2017

Academic Editor: Caigan Du

Copyright © 2017 Mingming Lv et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Semen Strychni have been shown to have therapeutic effect in improving blood circulation, relieving rheumatic pain, and treating cancer. However, Semen Strychni could cause severe nephrotoxicity. The present study was designed to evaluate whether treatment with total glucosides from paeony (TGP) has renoprotective effect against nephrotoxicity induced by total alkaloids from Semen Strychni (TAS). The levels of blood urea nitrogen (BUN) and creatinine (Cr) were determined and histopathological changes were also examined to evaluate renal injury. Moreover, a HPLC-MS method was developed and validated to investigate the comparative toxicokinetics of strychnine and brucine in rats plasma after oral administration of TAS and pretreatment with TGP. Results demonstrated that the levels of BUN and Cr were significantly increased () in TAS group, together with tubule epithelium cloudy swelling, degeneration, and glomerular atrophy in rats’ kidneys. The TAS-induced kidney damage was alleviated after pretreatment with TGP. Besides, of strychnine and brucine were increased and of strychnine and brucine were decreased after pretreatment with TGP. The toxicokinetics study showed that pretreatment with TGP could attenuate the absorption of strychnine and brucine, as well as accelerate their elimination. These results suggest that TGP possesses renoprotective effects.